294528.fig.002a
(a)
294528.fig.002b
(b)
294528.fig.002c
(c)
294528.fig.002d
(d)
Figure 2: (a) The most active stem bark extract was separated by Superdex G-75 column and resolved into five fractions (P1–P5). (b) The active fraction (P2) was further eluted and gave fractions CF-1–CF-F4. (c) The final pure fraction was separated by reverse-phase HPLC column (C8) using CF-1 fraction and designated as Calo-protein (Clo-p) of C. procera. Calo-p was tested for in vitro antimicrobial efficacy at various dose and in wound healing studies in mouse model. (d) The molecular mass of the Calo-protein was determined by MALDI-TOF/MS.