Pilot Study with regard to the Wound Healing Activity of Protein from Calotropis procera (Ait.) R. Br.
Figure 2
(a) The most active stem bark extract was separated by Superdex G-75 column and resolved into five fractions (P1–P5). (b) The active fraction (P2) was further eluted and gave fractions CF-1–CF-F4. (c) The final pure fraction was separated by reverse-phase HPLC column (C8) using CF-1 fraction and designated as Calo-protein (Clo-p) of C. procera. Calo-p was tested for in vitro antimicrobial efficacy at various dose and in wound healing studies in mouse model. (d) The molecular mass of the Calo-protein was determined by MALDI-TOF/MS.
