Pilot Study with regard to the Wound Healing Activity of Protein from Calotropis procera (Ait.) R. Br.
Table 1
Antimicrobial activity of different extracts of stem-bark of C. procera tested on Gram-positive and Gram-negative bacteria at 100 g/mL concentration and their cytotoxic potential was evaluated against human macrophage (U-937) cell lines by XTT assay at 2000, 1000, 500, 250, 100, 50, 25, and 12.5 g/mL doses.
Crude extracts
Phytochemical screening
Yield
E. coli
E. aerogenes
P. vulgaris
P. mirabilis
P. aeruginosa
S. aureus
Cytotoxicity (g/mL)
(1) Hexane
Flovanoids
5.36 gm
—
—
<250
(2) Ethyl acetate
Steroids
3.42 gm
—
—
<500
(3) Dichloromethane
Polyphenols
4.78 gm
—
—
<500
(4) Methanol
Terpenoids
5.98 gm
>2000
(5) Aqueous
Glycosides; Tannins; saponins
7.15 gm
>2000
(6) Chloramphenicol
Standard antibiotic
30 g
—
Clinical isolates of Gram-positive and Gram-negative bacteria were used. Values are mean SD of three replicates (), inhibition zones in diameters (mm) produced by the extract around the discs. Size of disc was 6 mm. Absence of bacterial inhibition denoted by (—), that is, no inhibition zone around the disc by crude extract of plants.