Antiangiogenic Effects and Therapeutic Targets of Azadirachta indica Leaf Extract in Endothelial Cells
Figure 1
Inhibition of endothelial cell tube formation with EENL treatment. HUVECs were seeded in a Matrigel-coated plate in the presence of 10–80 μg/mL of EENL or the vehicle control and VEGF (100 ng/mL). After incubation for 17 hours the cells were labeled with Calcein solution and tube images were taken at 2.5x magnification. The experiments were repeated twice in triplicate and the representative tube images were shown. (a) Vehicle control treated cells. (b) Cells treated with 10 μg/mL of EENL. (c) Cells treated with 20 μg/mL of EENL. (d) Cells treated with 40 μg/mL of EENL. (e) Cells treated with 60 μg/mL of EENL. (f) Cells treated with 80 μg/mL of EENL. (g) Tube length was measured using Image pro 6.2 software and the mean ± SD of two experiments with each performed in triplicate were shown (*).
