MART-10, a New Generation of Vitamin D Analog, Is More Potent than 1α,25-Dihydroxyvitamin D3 in Inhibiting Cell Proliferation and Inducing Apoptosis in ER+ MCF-7 Breast Cancer Cells
Figure 1
VDR expression in MCF-7 cells and MDA-MB-231 cells and the antiproliferative activity of 1α25(OH)2D3 and MART-10 in MCF-7 cells. (a) VDR expression in MCF-7 (lanes 1, 3, and 5) and MDA-MB-231 cells (lanes 2, 4, and 6) as determined by western blot method. Sixty μg proteins were added in each lane. VDR was expressed much more prominently in MCF-7 than in MDA-MB-231 cells. (b) The dose-dependent inhibitory effects of 1α25(OH)2D3 and MART-10 on the growth of MCF-7 cells. Cells were plated at 5,000 cells per cm2 in 35 mm dishes. Two days after plating, cells were treated with 1α25(OH)2D3 or MART-10 for 1 week at the indicated concentrations as described in the Materials and Methods. Cell numbers were obtained using a hemocytometer. Results are presented as the percentage of control. Each value is a mean ± SD of three to five determinations. , versus control. (c) The time-dependent inhibitory effects of 1α25(OH)2D3 and MART-10 on the growth of MCF-7 cells. Cells were grown and treated with 1α25(OH)2D3 or MART-10 at the indicated concentrations two days after plating and counted on days 3, 5, and 7, respectively. Cell numbers were obtained using a hemocytometer. Results are presented as the percentage of control. Each value is a mean ± SD of three to five determinations. , versus control. (d) The dose-response effects of 1α,25(OH)2D3 and MART-10 treatment on the growth of MDA-MB-231 cells. Cells plated at 5,000 cells per cm2 in 35 mm dishes were grown and treated with 1α,25(OH)2D3 or MART-10 at indicated concentrations for 1 week two days after plating as described in the Materials and Methods. Cell numbers were obtained using a hemocytometer. Results are presented as the percentage of control. Each value is a mean ± SD of three to five determinations. , versus control.
