MART-10, a New Generation of Vitamin D Analog, Is More Potent than 1α,25-Dihydroxyvitamin D₃ in Inhibiting Cell Proliferation and Inducing Apoptosis in ER+ MCF-7 Breast Cancer Cells

Figure 2

Flow cytometry analysis of cell cycle distribution for MCF-7 cells treated by 1α25(OH)₂D₃ and MART-10. Effects of 1α25(OH)₂D₃ and MART-10 on the relative distribution of MCF-7 cells at G₁/G₀, S and G₂/M phase. MCF-7 cells were treated with 1α25(OH)₂D₃ from 10⁻⁸ M to 10⁻⁶ and MART-10 from 10⁻⁹ M to 10⁻⁷ M for two days before cell cycle analysis was performed with a flow cytometer. A representative DNA histogram for control, 1α25(OH)₂D₃-, or MART-10-treated MCF-7 cells was shown. The total DNA content of cells (x-axis) was obtained by staining with propidium iodide. Cells were analyzed by flow cytometry. The percentage of cells in each cell cycle phase was determined with the program ModFit. The first large peak represents population of cells (y-axis) in G₀/G₁ phase, the second small peak shows population of cells in G₂/M phase, and the gray area between both peaks represents cells in S phase.