Research Article

MART-10, a New Generation of Vitamin D Analog, Is More Potent than 1α,25-Dihydroxyvitamin D3 in Inhibiting Cell Proliferation and Inducing Apoptosis in ER+ MCF-7 Breast Cancer Cells

Figure 5

Effects of 1α25(OH)2D3 and MART-10 on the protein expression of Bcl-2, Bax, and cytochrome C in MCF-7 cells. (a) Western blot analysis of Bcl-2 and Bax expression in the untreated control, and cells treated with either 10−7 M of 1α25(OH)2D3 or MART-10 for 5 days (upper panel). Thirty μg protein was loaded in each lane. The lower panel depicts the calculated BAX/Bcl-2 ratio obtained from scanning the bands shown in the upper panel based on the control Bax/Bcl-2 ratio set as 1. (b) Western blot analysis of cytochrome C expression in cytosol after treatment with ethanol vehicle, 1α25(OH)2D3, or MART-10 (upper panel) and the expression ratio over the control (lower panel). Each value represents the average of three determinations. , versus control.
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