Research Article

Reversal of the Caspase-Dependent Apoptotic Cytotoxicity Pathway by Taurine from Lycium barbarum (Goji Berry) in Human Retinal Pigment Epithelial Cells: Potential Benefit in Diabetic Retinopathy

Figure 6

Caspase-3 activity in high-glucose-treated ARPE-19 cells. The effect of LB extract and taurine on fluorometrically determined caspase-3 activity was measured in lysates of high-glucose-treated ARPE-19 cells. The -axis shows the amount of free 7-amino-4-methylcoumarin (AMC) released from the caspase-3-specific substrate DEVD (N-acetyl-Asp-Glu-Val-Asp)-AMC. Levels in the control were arbitrarily assigned a value of 1.0. All values are means ± SEM ( , each in duplicate). # versus control, * , ** versus HG.
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