Hwangryun-Haedok-Tang Fermented with Lactobacillus casei Suppresses Ovariectomy-Induced Bone Loss
Figure 3
fHRT-BU inhibits RANKL-induced NFATc1 expression in BMM cells. (a) BMMs (3 × 105 cells/well in a 6-well plate) were incubated with HRT-BU (10 μg/mL) and RANKL (150 ng/mL) or fHRT-BU and RANKL for the indicated times. Total RNA was isolated on the indicated time and mRNA expression of NFATc1, c-fos, TRAP, ATPv0d2, and cathepsin K was analyzed by QPCR. (b) Whole cell lysates (30 μg) were analyzed by western blot analysis with antibody specific for NFATc1 and c-fos. β-actin was used as loading control. Data are representative of three independent experiments.
