Hwangryun-Haedok-Tang Fermented with Lactobacillus casei Suppresses Ovariectomy-Induced Bone Loss
Figure 4
fHRT-BU inhibits RANKL-induced p38, IKKα/β, and p65 phosphorylation in BMM cells. (a) BMMs (3 × 105 cells/well in a 6-well plate) were pretreated with HRT-BU or fHRT-BU (10 μg/mL) for 2 h and then stimulated with RANKL (150 ng/mL) for the indicated times. Whole cell lysates (10 μg) were analyzed by western blot analysis with indicated antibodies. MAP kinases, IKKα/β, and p65 activation was represented by the levels of protein phosphorylation. β-actin was used as loading control. (b) BMMs were pretreated with HRT-BU or fHRT-BU (10 μg/mL) for 2 h and then stimulated with RANKL (150 ng/mL) for 3, 6, and 12 h. Total RNA was isolated on the indicated time and mRNA expression of ICAM-1, TNF-α, Nfκb2, and I-κB was analyzed by QPCR. Data represents mean ± SD of three independent experiments.
