Figure 9: The effect of ACR and SN pretreatment on the protein level of cytochrome c in cytosolic fraction in heart of mice. (a) Representative western blots indicating specific bands for cytochrome c and β-actin as an internal control. Equal amounts of protein sample (50 μg) obtained from the cytosolic fraction homogenate were applied in each lane. These bands are representative of six separate experiments. (b) Densitometric data of protein analysis. Vehicle-control group received normal saline +0.5% w/v methylcellulose, orally for 3 weeks. ACR was given (7.5 mg/kg, orally) for 3 weeks. SN was injected 7 days before ACR and daily thereafter throughout the study (3 weeks). Vit E (100 IU/Kg, three times per week) plus ACR were administrated for 3 weeks. SN alone at dose (100 mg/kg, intraperitoneally) was treated for 4 weeks. Data are expressed as the mean ± SEM. *** versus vehicle-control group, ### versus ACR group.