Wnt-Signaling-Mediated Antiosteoporotic Activity of Porcine Placenta Hydrolysates in Ovariectomized Rats

Figure 2

Femur relative mRNA levels of the Wnt signaling proteins; LRP5, β-catenin, DKK1, and RANKL. Ovariectomized (OVX) rats were divided into four groups, and for 12 weeks each group was orally administered 1 g/kg bw/day of porcine placenta hydrolysates with (WPPH) or without ovarian hormones (NPPH), 0.1 mg/kg bw/day conjugated estrogen (EST), or dextrose (OVX-control), while Sham rats were orally administered 1 g/kg/day dextrose as a normal control (Sham-control). After the experimental period, mRNA levels in the femur were measured by real-time PCR. Values are expressed as means ± SD (𝑛=5). ^∗Significantly different among the treatments at 𝑃<0.05. ^a,b,cSignificantly different among the OVX groups by Tukey’s test at 𝑃<0.05. ^†Significantly different between the OVX-control and Sham-control groups at 𝑃<0.05.