Xiaoyaosan Decoction Regulates Changes in Neuropeptide Y and Leptin Receptor in the Rat Arcuate Nucleus after Chronic Immobilization Stress
Figure 3
Double immunofluorescence for NPY and ob-R in the ARC. (A–D) Representative images of NPY, ob-R, and colocalization in ARC. (A) The control group. (B) The 7-day stress group. (C) The 21-day stress group. (D) The Xiaoyaosan-treated group. In each figure, ob-R was labeled with green fluorescence (I), NPY was labeled with red fluorescence (II), and co-loalization of NPY and ob-R was double-labeled in yellow (III). Scale bars = 50 μm for all images. NPY and Ob-R colocalization area, NPY or Ob-R IOD, and weighted colocalization coefficient were analyzed with Zeiss LSM Image Examiner, and the number of positive neurons was measured with Image Pro Plus. Each bar represents the mean values and vertical bars represent SEM. ▲ as compared with the 21-day stress group. 3V means the third ventricle.
