Pomegranate Protection against Cardiovascular Diseases
Figure 5
Pomegranate juice (PJ) or whole pomegranate fruit extract (POMxl) increase PON1 binding to HDL. Twenty patients with type 2 diabetes mellitus participated in the study. Ten male patients received concentrated PJ (50 mL/day for 4 weeks) while another group of 10 male patients received POMxl (5 mL/day for 6 weeks). (a) Blood samples were collected from both groups before (0 time) and 4 weeks after PJ consumption, or (b) 4 weeks after POMxl consumption. The HDL or LPDS fractions were isolated from the blood samples of 4 patients by density gradient ultracentrifugation. The HDL fractions (25 μg protein) or LPDS fractions (20 μL) were loaded on 10% acrylamide gel, and PON1 protein bands were visualized using mouse anti-human PON1 antibody. PON1 bands and densitometric analysis of the PON1 bands are shown. This is a representative experiment out of four. * versus time 0.
