Pomegranate Protection against Cardiovascular Diseases
Figure 6
Pomegranate Juice (PJ) polyphenols upregulated paraoxonase 1 (PON1) expression in hepatocytes, via PPARγ, resulting in the secretion of biologically active PON1. (a) PON1 mRNA expression in human hepatoma cell line (HuH7) that were incubated with increasing concentrations (17–70 μg of gallic acid equivalents (GAE)/mL) of PJ, or its polyphenols: punicalagin, gallic, or ellagic acids, for 24 hours at 37°C. (b) PON1 mRNA expression in HuH7 cells, untreated (Control) or treated with pomegranate or its major polyphenols, in the absence or presence of the PPARγ antagonist GW9662 (50 μmol/L). (c) Secreted PON1 arylesterase activity was measured in the medium from control cells, or from pomegranate polyphenols-treated cells in the presence of HDL from PON1 knockout (KO) mice. (d) LDL was oxidized by copper ions in presence of conditioned medium from untreated (Control-CM) and from gallic acid treated cells (Gallic acid-CM). Results are expressed as mean ± SD of three different experiments. * versus control.
