Inhibition of Hypoxia Inducible Factor Alpha and Astrocyte-Elevated Gene-1 Mediates Cryptotanshinone Exerted Antitumor Activity in Hypoxic PC-3 Cells
Figure 1
Effect of cryptotanshinone (CT) on hypoxia-induced HIF-1α activation in PC-3 cells. (a) Chemical structure of CT. (b) Effect of CT on the viability of prostate cancer cells under normoxia and hypoxia. (c) Effect of HIF-1α accumulation in hypoxic PC-3 cells. Cells were treated with CT (10 μM) for 0.5, 2, 4, 6, and 24 h under hypoxia and Western blotting was performed. (d) Effect of CT on HIF-1α transcriptional activity in hypoxic PC-3 cells by ELISA. Cells were treated with or without CT (5 or 10 μM) under normoxia or hypoxia for 6 h and HIF-1α transcriptional activity was evaluated by TransAM HIF-1 transcriptional factor assay kit. Data represent means ± S.D. ## versus normoxia control. * or ** versus hypoxia control. (e) Effect of CT on HIF-1α nuclear translocation in hypoxic PC-3 cells. Cells treated with or without CT (10 μM) under hypoxia for 6 h were fixed with 10% formalin for immunocytochemistry fluorescence staining. Green color was detected for HIF-1α in PC-3 cells, while nuclei were counterstained with blue color using DAPI.
