Inhibition of Hypoxia Inducible Factor Alpha and Astrocyte-Elevated Gene-1 Mediates Cryptotanshinone Exerted Antitumor Activity in Hypoxic PC-3 Cells
Figure 2
CT attenuates the expression of AEG-1 and HIF-1α in hypoxic PC-3 cells. (a) Expression of AEG-1 in various prostate cancer cells and normal prostate cells under hypoxia. Cell lysates were prepared and subjected to Western blotting. (b) Effect of hypoxia (left panel) or CT (right panel) on the expression of AEG-1 and HIF-1α in hypoxic PC-3 cells. (c) Effect of AEG-1 or HIF-1α siRNA transfection on AEG-1 expression by Western blotting (left panel) or HIF-1α activity by ELISA (right panel). (d) Effect of DMOG on the expression of AEG-1 and HIF-1α in hypoxic PC-3 cells. (e) Effect of PI3K inhibitor wortmannin (20 μM) on AEG-1 or HIF-1α expression under normoxia and hypoxia by Western blotting.
