Demethoxycurcumin Retards Cell Growth and Induces Apoptosis in Human Brain Malignant Glioma GBM 8401 Cells
Figure 2
Reduction of the mitochondrial membrane potential (MMP) in the GBM 8401 cells by DMC, which was determined by Rhodamine 123 and JC-1 staining and detected by fluorescence microscopy, fluorescence reader, and flow cytometry: (a) MMP is shown to be significantly reduced in the GBM 8401 cells treated with DMC (12.5, 25, and 50, μM) by Rhodamine 123 staining, and the same effect was also demonstrated by JC-1 staining (c). (b) Using BioTek FLx800 TBI fluorescence reader, the intensity of fluorescence was determined and found to decline (presented as the percentage of the controls) as the concentration of DMC used to treat the GBM 8401 cells increased. All the data shown are the mean (±SEM) of at least three independent experiments. The symbol (*) on each group of bars denotes that difference from the treatment with 0 μM DMC is statistically significant at .
