Apoptosis Induction in Primary Human Colorectal Cancer Cell Lines and Retarded Tumor Growth in SCID Mice by Sulforaphane
Figure 6
SFN activates procaspase-3 degradation in five CRC cell lines. The cells were treated with SFN (0, 6.25, 12.5, and 25 μM) for 24 hours, and then Western blot analysis was performed for procaspase-3. (a) Representative blot from 3 independent experiments. (b) Quantification of band intensities by Li-COR near infrared imaging system. (c) The caspase-2, -3, -8, -9 activity was analyzed by ApoAlert Caspase assay plates. SFN induces the caspase activity of five CRC cell lines. (d) Quantification of DNA fragmentation by measuring the fluorescence intensities by flow cytometry. The data showed that DNA fragmentation levels were significantly elevated in cells incubated with SFN incubation for 24 hours. All data were reported as the means (±SEM) of five CRC cell lines. Statistical analysis used the t-test, with the significant differences determined at the level of *P < 0.05 versus 0 μM control group.
