Anti-Inflammatory Activities of Cinnamomum cassia Constituents In Vitro and In Vivo
Figure 4
Inhibition of NF-κB and IκBα (a) protein expressions by Cinnamomum cassia constituents (cinnamic aldehyde, cinnamic alcohol, cinnamic acid, and coumarin) in LPS-stimulated RAW264.7 cells. Samples (50 μM) were added into cells 1 h before LPS (100 ng/mL) stimulation and protein samples were prepared for 1 h after LPS stimulation. Activations of signaling molecules were then evaluated by Western blot analysis. Lysed cells were then prepared and subjected to western blotting using an antibody specific for NF-κB (P65) and IκBα in the cytosol. β-actin was used as an internal control. A representative western blot from two separate experiments is shown. Relative NF-κB and IκBα protein levels were calculated with reference to an LPS-stimulated culture (b). ###compared with sample of control group. The data were presented as mean ± S.D. for three different experiments performed in triplicate. * and *** were compared with LPS-alone group.
