A Novel Herbal Medicine KIOM-MA Exerts an Anti-Inflammatory Effect in LPS-Stimulated RAW 264.7 Macrophage Cells
Figure 5
Effect of KIOM-MA on LPS-induced (a) RAW 264.7 cell migration, (b) activity and expression of MMP-9. Cells were pretreated with indicated concentrations of KIOM-MA for 30 min before LPS stimulation. After incubation for 48 hours, (a) cells on lower filter surfaces were fixed and stained with 20% methanol/0.2% crystal violet (v/v). Migrated cells were determined under microscope by counting cells in 5 random fields. Data shows mean ± SD values from three independent experiments. * and ** were calculated from comparing with LPS-stimulation value. (b) The culture supernatant of RAW 264.7 cell was prepared and used for gelatin zymography and Western blot analysis. The experiment was repeated three times independently.
