A Study of the Wound Healing Mechanism of a Traditional Chinese Medicine, Angelica sinensis, Using a Proteomic Approach
Figure 2
Differential effect of AS and FA treatment on human fibroblasts as visualized by 2D PAGE. 300 μg/mL AS extract and 3.5 μM FA were used to treat cells for 24 hr. Whole-cell lysates were extracted and analyzed by 2D PAGE (first dimension, 18 cm, pH 4–7, nonlinear gradient of IPG strips; second dimension, 12.5% SDS-PAGE) and visualized by silver staining. There were 29 and 22 differentially expressed spots that could be identified from the AS extract and FA-treated cells, respectively, compared with the DMSO control. The numbered protein spots were identified by LC-MS/MS mass spectrometry. The corresponding protein spot identities are shown in Table 1.
