A Study of the Wound Healing Mechanism of a Traditional Chinese Medicine, Angelica sinensis, Using a Proteomic Approach

Figure 3

Hierarchical clustering and functional classification of the changes in protein expression between the DMSO control cells and AS/FA-treated cells. The expression pattern for each protein was categorized by UPGMA using Hierarchical Clustering Explorer 3.5. Proteins with a similar expression clustered into five different discrete groups (clusters A, B, C, D, and E) in a tree-like organization. Each row in the color heat map indicates a single protein and each column represents different groups of proteins from the DMSO, AS, and FA treatments. A high protein expression value for a specific protein spot is represented by a bright red color, and a low protein expression value is represented by a bright green color. A black color indicates that the protein spot was expressed at an average level. A gray color represents protein spots that were undetectable. Further information about the differentially expressed protein spots that were identified is given in Table 1. The functional classification of each protein was determined using BGSSJ and the Swiss-Prot protein sequence database.