A Study of the Wound Healing Mechanism of a Traditional Chinese Medicine, Angelica sinensis, Using a Proteomic Approach
Figure 4
Analysis of the results of 2D PAGE by immunoblotting and ROS assay. According to hierarchical clustering and functional classification, the expressions of GSTP1 and TPIS were both increased in 300 μg/mL AS extract and 3.5 μM FA-treated cells. In addition, STMN expression increased in AS-extract-treated cells, and HSPB1 expression increased in FA treated cells. GSTP1 expression was examined by immunoblotting (a) using anti-GSTP1 antibody and these results were then further confirmed by functional ROS assay (e). The expression of TPIS (b), HSBP1 (c), and STMN (d) were also confirmed by immunoblotting using specific antibodies. In all cases the results correlated with the clustering and functional classification of 2D PAGE. β-Tubulin was employed as the sample loading control. The relative expression levels were detected by Fujifilm Multigauge ver. 2.0 for densitometric analysis and normalized with internal control, and a Student’s test was performed to evaluate the statistical significance. Results are presented as mean ± SD (). *, # but with a decreased trend compared with DMSO.
