Chemical Composition, Toxicity and Vasodilatation Effect of the Flowers Extract of Jasminum sambac (L.) Ait. “G. Duke of Tuscany”

Table 1

TLC Method.

Test	Standard	Mobile phase	Spraying reagent	Detection	Color
Alkaloids	Atropine	Toluene : ethyl acetate :  dimethylamine (70 : 20 : 10)	Dragendorff reagent	Visible	Orange spot on yellow background
Anthraquinones	Rhein	Ethyl acetate :  methanol : water (81 : 11 : 8)	KOH reagent	UV 366 nm	Yellow/orange/red
Antioxidants	Vit E	Ethyl acetate : toluene (50 : 50)	DPPH reagent	Visible	White spot on purple background
Coumarins	Coumarin	Toluene : ethyl acetate (90 : 10)	KOH reagent	UV 366 nm	Light blue/green
Essential oils	Eugenol	Toluene : ethyl acetate (93 : 7)	Anisaldehyde sulfuric acid reagent	Heat 100°C Visible	Purple/red
Flavonoids	Rutin	Ethyl acetate : formic acid : acetic acid : water (100 : 11 : 11 : 27)	Natural Product (NP/PEG)	UV 366 nm	Orange/yellow/green/blue
Cardiac glycosides	Ouabain	Ethyl acetate : methanol : water (81 : 11 : 8)	Kedde reagent	Visible	Pink/yellow/purple
Phenolics	Catechol	Toluene : ethyl acetate (97 : 3)	Ferric chloride reagent	Visible	Blue/black
Saponins	Saponin	Butanol : ethyl acetate : acetic acid : water (10.8 : 3.6 : 0.2 : 2.7)	Anisaldehyde sulfuric acid reagent	Visible	Blue
Tannins	Gallic acid	Metanol : ethyl acetate : acetic acid (10 : 90 : 0.1)	Ferric chloride reagent	Visible	Blue/black