Ginseng Berry Extract Prevents Atherogenesis via Anti-Inflammatory Action by Upregulating Phase II Gene Expression
Figure 7
KGBE suppresses adhesion molecule expression in TNF-α-stimulated endothelial cells and aortas from mice fed a HFD. (a) HUVECs were pretreated with KGBE in the presence or absence of SnPP and BSO for 30 min. The cells were stimulated with 10 ng/mL TNF-α for 12 h. The protein levels of ICAM-1 and VCAM-1 were determined by Western blotting. (b) TNF-α-stimulated HUVECs were cocultured with fluorescence-labeled U937 cells for 1 h. After washing, adherent monocytes were photographed by fluorescence microscopy. (c) Monocytes bound to HUVECs were lysed, and fluorescence intensity was determined using a florescence plate reader. Data shown are the mean ± S.D. (). **. (d) Levels of ICAM-1 and VCAM-1 mRNAs were determined in aortic tissues from ApoE−/− mice fed NCD, HFD, and HFD supplemented with KGBE for 16 weeks.
