Effects and Interaction of Icariin, Curculigoside, and Berberine in Er-Xian Decoction, a Traditional Chinese Medicinal Formula, on Osteoclastic Bone Resorption

Figure 5

Effects of I, B, C, and their combination on expression of OPG and RANKL of osteoblasts. Primary osteoblastic cells from neonatal rat calvaria were treated with or without I, B, C, and their combination for 24 h. (a) The ratio of protein expression of OPG and RANKL. (b) Protein expression of OPG and RANKL was determined by Western blotting. β-actin was used as an internal reference. Data were presented as mean ± standard deviation. The experiments were repeated 3 times in five replicate samples (𝑛=5), ^∗𝑃<0.05, ^∗∗𝑃<0.01, ^∗∗∗𝑃<0.001 compared with control.

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