Figure 3: Western blot (a) and densitometric analysis for NMDAR NR2B subunit (b), PI3K (c), and CREB (d) in the L4-5 segment of the spinal cord in MK801- and EA-treated rats. The level of each protein is expressed as a percentage of the control. Each single experiment was conducted on three pooled animals. The panel represents a typical result from three independent experiments. EA-induced phosphorylation of NR2B subunit and PI3K was not observed by MK801 pretreatment, and a significant lower phosphorylation of CREB was detected from at 60 min after EA stimulations; .