Figure 5: Western blot (a) and densitometric analysis (b) for induction of total and phosphorylation of PI3K and CREB in the L4-5 segment of the spinal cord in TMB8- and EA-treated rats. The level of each protein is expressed as a percentage of the control. Each single experiment was conducted on three pooled animals. The panel represents a typical result from three independent experiments. EA-induced phosphorylation of PI3K and CREB was arrested by TMB8 pretreatment. .