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Figure 6: Immunohistochemical localization of pPI3K in the dorsal horn L4-5 segment in normal and EA-treated rats. The expression of pPI3K (red) was usually detected in the laminae IV-VI regions of dorsal horn of the spinal cord. NeuN (green) was also detected in the similar region, and merged image showed that pPI3K and NeuN were colocalized. Scale bar = 200 μm.