501796.fig.003a
(a)
501796.fig.003b
(b)
501796.fig.003c
(c)
Figure 3: ISCADOR does not induce cell death at a concentration of 100 μg/mL. (a) LNT-229 cells were treated with ISCADOR (100 μg/mL, 24 h), or were left untreated, and then allowed to grow. Cell density was assessed by crystal violet staining every 24 h ( for LNT-229 cells, for SMA560 cells, SEM). (b) The cells were treated as in (a). Viable cells were determined by trypan blue staining ( , SEM). (c) Fluorescence based Caspase 3/7 activity assay. The cells were treated as indicated and caspase activity was analyzed 16, 24 and 48 h later using the fluorescent substrate DEVM-amc. As a positive control, the cells were treated with CD95L (100 U/mL + 10 μg/mL CHX) for 4 h ( , SEM).