501796.fig.006a
(a)
501796.fig.006b
(b)
501796.fig.006c
(c)
501796.fig.006d
(d)
Figure 6: ISCADOR Q mediated reduction of cell motility is caused by reduced MMP-expression and activity. (a) LNT-229 cells were treated with ISCADOR Q or were left untreated. 24 h later, cellular supernatants (SN) or lysates were prepared. MMP or TIMP expression was analyzed by immunoblot (Ab; specific antibody, one representative experiment is shown). (b) Quantification of protein expression ( for MMP-1, for MMP-2, for MMP-3, for MMP-9, for MMP-10, for MMP-14, for TIMP-2). (c) Gelatinase zymogram analyzing MMP activity ( , one representative experiment is shown). (d) Quantification of MMP gelatinase activity ( for MMP-9, for MMP-2, for 36 kD active MMP-2; SEM). Note that the activated form of MMP-9 could not be separated strictly from MMP-2. Therefore quantification of MMP-9 activity was only done for proMMP-9.