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(a)
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(b)
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(c)
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(d)
Figure 7: ISCADOR Q reduces the growth of subcutaneously growing GBM in mice. (a) and (b) The cells were pretreated with ISCADOR Q (100 μg/mL) or were left untreated. One million viable LNT-229 cells were implanted into the right flank of nude mice (a), or one million SMA560 cells into VMDk-mice (b) Tumor size was measured 3 times per week. Inlet: As a control and to avoid the measurement of artifacts induced by ISCADOR Q mediated inhibition of cell growth, proliferation of implanted cells was assessed by crystal violet staining in parallel. (c) One million LNT-229 cells were implanted into the right flank of nude mice and tumors were allowed to grow for six days. At day 7 subcutaneous injections twice a week with PBS or a weekly increasing dose of ISCADOR Q (1 up to 100 μg in total, arrows indicate ISCADOR injections) were given on the contralateral body site. After two weeks of treatment with the highest dosage, injections were stopped and tumor size was measured as in (a). (d) One million SMA560 cells were implanted into the right flank of VMDk mice and tumors were allowed to grow for six day. On day 7, a single intratumoral injection of ISCADOR Q (20 μL, 100 μg/mL, indicated by an arrow) was given into the palpable tumors. Tumor size was measured as in (a).