Botanical Extracts from Rosehip (Rosa canina), Willow Bark (Salix alba), and Nettle Leaf (Urtica dioica) Suppress IL-1𝜷-Induced NF-κB Activation in Canine Articular Chondrocytes

Figure 1

Effect of botanical extracts and IL-1β on the proliferation of chondrocytes in vitro. Serum-starved chondrocytes were exposed to IL-1β (10 ng/mL) for 48 h, botanical extracts (10 μg/mL) for 72 h, cotreated first with botanical extracts (10 μg/mL each) for 24 h, and then with IL-1β (10 ng/mL) for 48 h, treated with DMSO (as control) for 72 h or left untreated for 72 h. Cell viability was examined by MTT assay. The MTT assay is a spectrophotometric measurement of the cell viability as a function of the mitochondrial activity. This assay was performed in triplicate and the results are provided as mean values with standard deviations from three independent experiments. Treatments: Untreated control; IL-1β; RC (Rosa canina); SA (Salix alba); UD (Urtica dioica).