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Evidence-Based Complementary and Alternative Medicine
Volume 2012 (2012), Article ID 535479, 8 pages
http://dx.doi.org/10.1155/2012/535479
Research Article

Acetone Extract from Rhodomyrtus tomentosa: A Potent Natural Antioxidant

1Natural Products Research Centre, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand
2Department of Microbiology, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand
3Department of Biochemistry, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand

Received 19 April 2012; Accepted 18 June 2012

Academic Editor: Vassya Bankova

Copyright © 2012 Goodla Lavanya et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Rhodomyrtus tomentosa (Myrtaceae) has been employed in traditional Thai medicine to treat colic diarrhoea, dysentery, abscesses, haemorrhage, and gynaecopathy. In addition, it has been used to formulate skin-whitening, anti-aging and skin beautifying agents. Ethnomedical activities of this plant may be due its antioxidant property. Hence, the aim of this study was to evaluate both in vitro and in vivo antioxidant activities of R. tomentosa leaf extract. In vitro antioxidant activity of the extract was assessed by lipid peroxidation inhibition capacity, ferric reducing antioxidant power, and metal chelating activity. R. tomentosa extract demonstrated its free radical scavenging effects in concentration dependent manner. In vivo antioxidant activity of the extract was conducted in Swiss Albino mice. Levels of thio-barbituric acid reactive substances (TBARS), glutathione (GSH), and the activities of antioxidant enzymes including superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) in blood, liver, and kidney were analyzed using microtitre plate photometer. Administration of CCl4 caused significant increase in TBARS and decrease in GSH, SOD, CAT and GPx levels. In contrast, R. tomentosa extract (0.8 g/kg) effectively prevented these alterations and maintained the antioxidant status. The results suggest that R. tomentosa extract can serve as a potent antioxidant.