Neutralisation of Local Haemorrhage Induced by the Saw-Scaled Viper Echis carinatus sochureki Venom Using Ethanolic Extract of Hibiscus aethiopicus L.
Figure 1
(A) Dissected skins of guinea pigs showing dose dependent of antivenom neutralisation efficacy of H. aethiopicus extract. (a) Control: animals were injected with 75 μg of E. c. sochureki alone. (b–k) represent different concentration (100–1000 mg/kg) of the H. aethiopicus extract. (f) represents the optimum concentration (500 mg/kg) of the H. aethiopicus extract that gives a complete venom neutralisation after both venom and extract were incubated in a test tube for 30 minutes. (B) Response curve showing dose dependent of antivenom neutralisation efficacy of H. aethiopicus extract. Neutralisation efficacy of different concentrations (100–1000 mg/kg) of the H. aethiopicus extract preincubated in a test tube for 30 minutes with fixed concentration of the E. c. sochureki venom. Chart illustrates the end point concentration (i.e., 400) where no adequate haemorrhage pattern found to be measured. ** and ***.
