Topical Application of Herbal Mixture Extract Inhibits Ovalbumin- or 2,4-Dinitrochlorobenzene-Induced Atopic Dermatitis
Figure 1
Protocol for induction of atopic dermatitis. Shaved dorsal regions of the mice were sensitized epicutaneously with OVA (a) or DNCB (b) solution. (a) Female BALB/c mice were intraperitioneally sensitized with OVA once a week for three weeks. After three weeks, the back of the mice was shaved with an electric razor and dermally challenged with 1 1 cm sterile patches containing OVA (100 μg) for 3 weeks. KM110329 was applied to the skin during the second and third skin sensitization week together with OVA. (b) Male BALB/c mice were epicutaneously sensitized with 200 μL of a 1% DNCB solution on day 1. Two weeks later, dermatitis was induced with 200 μL of 0.2% DNCB solution at the intervals shown in figure. KM110329 was applied to the skin from the 3th week during sensitization together with DNCB.
