LC-MS/MS Identification of a Bromelain Peptide Biomarker from Ananas comosus Merr

Figure 1

Bromelain characterization flow diagram. A clinically used quality verified Br product (A) was processed and electrophoresed on 1D SDS PAGE mini gel (B). The mobility region was excised and gel digests were analyzed via LC-MS/MS for protein identification. Production data were searched against all kingdoms of the NCBInr database via Mascot search engine and files were parsed into the Scaffold proteome program (C). After i.p. Br treatment (D) plasma samples were assed via SRM for presence of Br specific peptides and DYGAVNEVK was identified and quantified over 24 hrs (E).