Refined Qingkailing Protects MCAO Mice from Endoplasmic Reticulum Stress-Induced Apoptosis with a Broad Time Window

Figure 5

Effect of RQKL on protein levels of caspase3, procaspase12, and P-elF2a in cortex of MCAO mouse. Mice were subjected to 1.5 h ischemia followed 22.5 h reperfusion. QKL or RQKL was injected immediately after ischemia. The tissue samples were from the cerebral cortex. The panels are representative Western blotting analyses of caspase3, procaspase12 and P-elF2a. The gray values were calculated, and protein levels were expressed by the ratio of aim protein/β-actin as mean ± SEM. Notes ^ΔΔ𝑃<0.01, versus normal, ^##𝑃<0.01, versus ischemia, ^#𝑃<0.05, versus ischemia, 𝑛=5. Primary antibodies were diluted, caspase12 1 : 400, caspase3 1 : 2000, and P-elF2a 1 : 500.

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