Inhibition of Connexin 26/43 and Extracellular-Regulated Kinase Protein Plays a Critical Role in Melatonin Facilitated Gap Junctional Intercellular Communication in Hydrogen Peroxide-Treated HaCaT Keratinocyte Cells

<table>Melatonin significantly enhanced the expression of Cx26 and Cx43 at mRNA and protein levels, but not that of Cx30 in H<sub>2</sub>O<sub>2</sub>-treated HaCaT cells. (a) Cells were exposed to H<sub>2</sub>O<sub>2</sub> (300 <i>μ</i>M) with or without melatonin (1 or 2 mM) for 24 h. (a) mRNAs expressions of Cx26, Cx30, and Cx43 were analyzed by RT-PCR. Grapes represent relative level of Cx26, Cx30, and Cx43/GAPDH. (b) Quantification of mRNAs expression. Phosphorylation of Cx43 (c) and protein expressions of Cx26, Cx30, and Cx43 (d) in melatonin-H<sub>2</sub>O<sub>2</sub>-treated cells were analyzed by western blot. (e) Grapes represent relative level of Cx26, Cx30, and Cx43/<i>β</i>-actin.</table>

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Figure 3: Inhibition of Connexin 26/43 and Extracellular-Regulated Kinase Protein Plays a Critical Role in Melatonin Facilitated Gap Junctional Intercellular Communication in Hydrogen Peroxide-Treated HaCaT Keratinocyte Cells 

Figure 3 | Inhibition of Connexin 26/43 and Extracellular-Regulated Kinase Protein Plays a Critical Role in Melatonin Facilitated Gap Junctional Intercellular Communication in Hydrogen Peroxide-Treated HaCaT Keratinocyte Cells 