Inhibition of Connexin 26/43 and Extracellular-Regulated Kinase Protein Plays a Critical Role in Melatonin Facilitated Gap Junctional Intercellular Communication in Hydrogen Peroxide-Treated HaCaT Keratinocyte Cells
Figure 5
Combined treatment of melatonin and vitamin C at low concentrations exerted the synergy in reducing ROS production in H2O2-treated HaCaT cells. H2O2-treated HaCaT cells were exposed in the absence or presence of melatonin (200 μM), vitamin C (10 μg/mL), and melatonin plus vitamin C for 24 h. (a) Western blotting was performed for Cx43 and β-actin. (b) ROS generation (%) was measured using ROS-sensitive fluorometric probe 2,7-dichlorofluorescein diacetate (DCFDA) by flow cytometric analysis. Graph represents quantification for ROS production.
