Early Blockade of TLRs MyD88-Dependent Pathway May Reduce Secondary Spinal Cord Injury in the Rats
Figure 4
Total apoptosis at 7 d after injury. Western blotting (a) showed that, compared with CP group, caspase-3 in the spinal cord was significantly decreased in MIP group. Levels of proteins expression were presented as the ratio of the bands area integral values under absorption curve of each protein with the area integral values of β-actin. The quantified data (b) are represented, for each group, , *. Immunohistochemistry for caspase-3 (green) and Hoechst 33342 (blue) counterstaining showed more apoptotic cells in CP group (c) than that in MIP group (d) regardless of cell types, and (f) was the higher magnification of the square in (c) showing that caspase-3 immunoreactive product appears in cytoplasm of the cells. The diagram (e) showed number of caspase-3 positive cells counted from 4 represented areas randomly chosen in the sections, and the number of apoptotic cells in MIP group was significantly decreased, compared with CP group (*).
