Curcumin Protects against UVB-Induced Skin Cancers in SKH-1 Hairless Mouse: Analysis of Early Molecular Markers in Carcinogenesis
Figure 1
Experimental design and effect of CUR on UVB-induced skin photocarcinogenesis in SKH-1 hairless mouse. Upper panel, experimental design depicting variables and treatment groups both in long-term and short-term study. Mice were divided into 5 groups, a, b, c, d, and e. Twenty mice per group for long-term study and 5 mice per group for short-term study. (a) UVR, UVB irradiation (180 mJ/cm2), twice/week for long-term and single exposure for short-term study. (b) CUR-T, topical application of CUR, twice/week for 26 weeks (long-term study); once for short-term study. (c) CUR-T + UV, topical application of CUR 30 min prior to UVB; UV + CUR-T, topical application of CUR immediately after UVB irradiation. Dorsal skins were topically wet dressed with a filter paper soaked with 10 mmol CUR in 200 mL acetone. Lower panels, effect of CUR on UVB-induced skin photocarcinogenesis in SKH-1 hairless mouse. The results were obtained from the long-term regimen shown in the upper panel. Experiment was terminated at 26 weeks after UVB exposure. Percentage of tumor incidence (a), tumor multiplicity per mouse (b), tumor volume per mouse (c), and body weight per mouse (d) were recorded and analyzed. The data shown in (c) were mean ± SE (bars). In each case, the data shown were from 20 mice in each group. No tumors were observed in control and topically treated CUR alone groups. versus UVB group.
