Inhibition of Advanced Glycation End-Product Formation by Origanum majorana L. In Vitro and in Streptozotocin-Induced Diabetic Rats

Table 2

The inhibitory effects of methanol extract of O. majorana on glycosylated protein glycated, hemoglobin GHb and HbA_1c.

Groups	GHb	HbA1c	Glycated protein (nmol/mg protein)
Negative control	8.9±0.06	7.9 ± 0.98	15.3±1.47
Positive control	27.6±1.34	17.5 ± 1.56	23.7±2.19
Methanol extract	18.6 ± 1.53^a	14.9 ± 1.25^a	19.1 ± 2.04^a
Glutathione	8.1 ± 0.08^a	9.0 ± 0.67^a	—
Aminoguanidine	—	—	20.2 ± 1.87^a

Negative control: incubation with hemoglobin (30 mg/mL), positive control: incubation with hemoglobin (30 mg/mL) + glucose (0.278 mM), methanol extract: incubation with hemoglobin (30 mg/dL) + glucose (0.278 mM) + methanol extract (10 mg/ mL), glutathione: incubation with hemoglobin (3 mg/dL) + glucose (2.7 mM) + glutathione (0.5 mM). Data as expressed as ± SD; ^a𝑃<0.05 versus positive control values.