Inhibition of Advanced Glycation End-Product Formation by Origanum majorana L. In Vitro and in Streptozotocin-Induced Diabetic Rats

Table 3

The prooxidant effect of amphotericin B (AB) at 5 and 10 𝜇M, and the antioxidant protection of 5 and 10 𝜇g/mL of OM against 10 𝜇M, AB-induced malondialdehyde (MDA) formation (nmol/mg LDL protein) and conjugated diene (CD) formation after a 72-h incubation at 37°C.

Groups	MDA formation; (nmol/mg LDL protein)	CD formation lag phase (min)
Control	13.26±2.43	7.8±2.60
AB 5 𝜇M	22.56 ± 5.19^a	46.7 ± 1.94^a
AB 10 𝜇M	34.29 ± 2.28^a	58.3 ± 2.87^a
AB 10 𝜇M + OM (5 𝜇g/mL)	17.31 ± 3.41^a	20.6 ± 3.28^a
AB 10 𝜇M + OM (10 𝜇g/mL)	11.56 ± 5.73^a	13.1 ± 1.76^a

Values are expressed as mean ± SD, ^asignificantly (𝑃<0.05) different from control, where the significance was performed by one-way ANOVA followed by post hoc Dunnett’s test.