Elimination of Cancer Stem-Like “Side Population” Cells in Hepatoma Cell Lines by Chinese Herbal Mixture “Tien-Hsien Liquid”

<table>Existence of SP cells in the established hepatoma cell lines. Hepatoma cell lines were plated one day before analysis to achieve approximately 30% confluence on the day of analysis. The cells were detached, labeled with the Hoechst 33342 in the presence or absence of 50 <i>μ</i>M verapamil and then analyzed by flow cytometry. The SP cells which disappeared in the presence of verapamil (lower panel) are outlined and shown as a percentage of the total cell population.</table>

Evidence-Based Complementary and Alternative Medicine

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Figure 1: Elimination of Cancer Stem-Like “Side Population” Cells in Hepatoma Cell Lines by Chinese Herbal Mixture “Tien-Hsien Liquid” 