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Evidence-Based Complementary and Alternative Medicine
Volume 2012 (2012), Article ID 623940, 9 pages
http://dx.doi.org/10.1155/2012/623940
Research Article

Analysis of Flavonoids from Eugenia uniflora Leaves and Its Protective Effect against Murine Sepsis

1Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CP 19046, 81531-980 Curitiba, PR, Brazil
2Departamento de Saúde Comunitária, Universidade Federal do Paraná, Rua Padre Camargo, 280, Alto da Glória, 80060-240 Curitiba, PR, Brazil

Received 5 September 2012; Revised 30 October 2012; Accepted 12 November 2012

Academic Editor: Juliano Ferreira

Copyright © 2012 Yanna D. Rattmann et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Eugenia uniflora, referred to as Pitanga cherry shrub, is largely distributed in tropical and subtropical America. This plant is cultivated in many countries and it is suitable for the production of juice, frozen pulp, and tea. Besides, it can be used as treatment for inflammatory diseases. We reported that a flavonoid-rich fraction (HE-Bu) obtained from leaves decreased the lethality induced by cecal ligation and puncture (CLP), a clinically relevant model of sepsis. The oral administration of HE-Bu reduced the late mortality rate by 30%, prevented neutrophil accumulation in lungs, decreased TNF-α and IL-1β serum levels, and markedly decreased iNOS and COX-2 protein expression by ileum cells. Chemical investigation showed myricetin and quercetin rhamnosides as the major components of this fraction. The results showed that HE-Bu protected mice from sepsis and indicated that this edible plant produces compounds that could be considered as potential adjuvants for sepsis treatment.