Research Article

Chloroform Fraction of Centratherum anthelminticum (L.) Seed Inhibits Tumor Necrosis Factor Alpha and Exhibits Pleotropic Bioactivities: Inhibitory Role in Human Tumor Cells

Figure 6

Effect of CACF on serum TNF levels in rats. Different treatment groups were pretreated i.p with 25, 50, and 100 mg/kg of (LPS + CACF), 6 mg/kg of dexamethasone (LPS + DEX) or with phosphate buffer saline (LPS + PBS), and DMSO (Untreated) for 30 mins. All the treatment groups were then either injected with 1 mg/kg of lipopolysaccharides (LPS) or with PBS for 90 mins. Blood was withdrawn, and serum TNF was quantified using ELISA. One-way ANOVA with Tukey’s post-analysis was used to calculate the statistical significance among the groups when compared to LPS + PBS. *** , ** , and * .
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