Chloroform Fraction of Centratherum anthelminticum (L.) Seed Inhibits Tumor Necrosis Factor Alpha and Exhibits Pleotropic Bioactivities: Inhibitory Role in Human Tumor Cells

Table 1

Antioxidant activity profile of the chloroform fraction of Centratherum anthelminticum (CACF).

Samples	DPPH IC50, μg/mL	FRAP (μmol/L)	ORAC Equivalent conc. Trolox (20 μg/mL (μM)** IC50		TPC μg GAE/mg
CACF	22.56^a  ± 1.4	1048.3^a   ±  21.2	992.34^a  ± 45.12	86.62	37.16±0.85
Ascorbic acid	15^a  ± 0.3	6240^b   ±  56.2	—	—	—
BHT	17^a  ± 0.4	907.7^a  ± 54.8	—	—	—
Quercetin	—	—	1018.00^b  ± 34.82	74.52	—

*The net AUC was calculated by subtracting the blank AUC from the AUC of each sample, the standards, and the positive control. Final ORAC values were expressed as the equivalent concentration of Trolox (TE) that gives the same level of antioxidant activity as the samples at 20 μg/mL. **Means with different alphabets are statistically significant.