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Evidence-Based Complementary and Alternative Medicine
Volume 2012 (2012), Article ID 638723, 6 pages
http://dx.doi.org/10.1155/2012/638723
Research Article

Metabolomics-Based Study of Clinical and Animal Plasma Samples in Coronary Heart Disease with Blood Stasis Syndrome

Department of Graduate School, Beijing University of Chinese Medicine, Beijing 100029, China

Received 14 November 2011; Revised 23 February 2012; Accepted 11 March 2012

Academic Editor: Keji Chen

Copyright © 2012 Huihui Zhao et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The aim of this study is to explore a bridge connecting the mechanism basis and macro syndromes of coronary heart disease with experimental animal models. GC-MS technique was used to detect the metabolites of plasma samples in mini swine models with myocardial infarction (MI) and patients with unstable angina (UA). 30 metabolites were detected in the plasma samples of more than 50 percent of model group and control group in swine, while 37 metabolites were found in the plasma samples of UA patients and healthy control group. 21 metabolites in the plasma samples of swine model and 20 metabolites in patients with UA were found of significant value. Among which, 8 shared metabolites were found of low level expression in both swine model and UA patients. Independent Student’s t-test, principal component analysis (PCA), and hierarchicalcluster analysis (HCA) were orderly applied to comprehend inner rules of variables in the data. The 8 shared metabolites could take place of the 21 or 20 metabolites in classification of swine model with MI and UA patients, which could be considered as a bridge connecting the mechanism basis and macrosyndromes of swine model with MI and UA patients.