Fisetin Inhibits Hyperglycemia-Induced Proinflammatory Cytokine Production by Epigenetic Mechanisms
Figure 1
The cytotoxicity of fisetin to cultured high glucose-induced THP-1 cells. (a) Chemical structure of fisetin. (b) Effect of fisetin on cell viability after 48 h was evaluated by the CCK-8 assay, as described in the methods. Human monocytic (THP-1) cells (1 × 105 cells/mL) were cultured in presence of osmolar control (14.5 mmol/L mannitol) or normal glycemic (NG, 5.5 mmol/L glucose) or hyperglycemic (HG, 20 mmol/L) conditions in absence or presence of fisetin (0, 3, 6, 10 μM) for 48 h as described in the methods and the media was collected. Results are shown as mean ± SD of five different experiments.
